StakMax. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The. VERSAmaxTM Tunable Microplate Reader Operator s Manual for Instruments with Serial Numbers Beginning. Measurement of brain-derived neurotrophic factor (BDNF) release. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. The conversion of L-DOPA to dopachrome was allowed to proceed for 40 min at 30 °C in a microplate reader (Versamax; Molecular Devices). A standard curve, provided by the manufacturer, was generated and the sample concentrations determined by 4-parameter logistic curve. TECHNICAL NOTE. To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. $495. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. Finally, 0. 4 Microplate Reader Software w WARRANTY. Cell viability was represented by percentage values compared to a. Reverse transcription-quantitative polymerase chain reaction To assess phospholipase C gamma 1 (PLCG1) and phospholi-pase C gamma 2 (PLCG2) mRNA levels in each. This user guide provides detailed instructions on how to operate, maintain, and troubleshoot the device, as well as examples of applications and. Tristar 3 Multimode Microplate Reader. Microwell plates, black (96-well) Rocking table with adjustable lifting frequency. FAST AND ACCURATE METERTECH Microplate Reader $3,650. The activity was measured at 40 °C following o-nitrophenol (ONP) release at 405 nm using a microplate reader (Versamax, Molecular Devices, San Jose, CA, USA). ). 1 M sodium phosphate buffer (pH 6. Table of Contents. printed in u. Microplate assays involved a 96-microplate reader (VERSAmax; Molecular devices, Sunnydale, California, USA) used with flat-bottomed 96-well microplates (NUNC, Sigma Aldrich, UK). 0 (Molecular Devices, Sunnyvale, CA). Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. BMG Labtech - THERMOstar. +49-89-9620-2340 Fax +49-89-9620-2345 Japan Nihon Molecular Devices Tel. Concluding Remarks. Molecular Devices VersaMax Microplate Reader. Spectrophotometers. 68 $. Select Start>Control panel>Device Manager. We would like to show you a description here but the site won’t allow us. Table 1 Conditions for biofilm formation on lids of 96-pin microtiter plates. With a wavelength. Find regional support. 2. In brief, mature 3T3-L1 cells were washed with DPBS, fixed with 10% formalin for 1 h at room temperature, washed once with 60%. The reaction was stopped with 100 µL of H 2 SO 4 1 M and quantified in a spectrophotometer (VERSAmax, Molecular Devices, San Jose, CA, USA) at 450 nm. g. The BMDM cells were plated into a 6-well culture plate at a density of 2. . ,, [28]. We would like to show you a description here but the site won’t allow us. 47 shipping. Concluding Remarks. 4. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. Formerly known as MicroMax, Molecular Devices’ unique SpectraDrop™ Micro-Volume Microplate, offers the highest throughput solution for low volume measurement available onthe market today. Absorbance was measured with a microplate reader (VERSAmax Molecular Devices Corporation, Sunnyvale, CA, USA) at 570 nm to assess cell viability. Cells were treated with 150 μl of DMSO and absorbance was quantified in 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). ISM Equipment Classification (Group 1, Class A) This equipment is designated as. VersaMax™ ELISA microplate reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 to 850 nm). However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. The activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). 5×10 3 cells were seeded in 96-well plates, treated with indicated inhibitors for 48 or 72 hours, analyzed 16 hours after BrdU addition, and absorbance measured at 450nm using a spectrophotometer plate reader (VersaMax, Molecular. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. VersaMax ELISA Microplate Reader. Monday to Friday 8am - 5pm GMT. The optical density (OD) of the mixture was measured at 490 nm with a microplate absorbance reader (VersaMax (Molecular Devices), Shanghai, China). The RT-qPCR analysis was performed on a 7500 Fast Real-Time PCR system (Applied Biosystems, USA). Measurement of biofilm formation inhibitory activity. The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). Sale Sold out. pdf file (03 Dec 2022) Manufacturer: Molecular Devices; Category of Device: Laboratory Equipment, Measuring Instruments; Document: versamax Operator's Manual, File Type: PDF;Search for used versamax. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. Molecular Devices also offers a mass spectrometry-based solution for food safety testing We would like to show you a description here but the site won’t allow us. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. VersaMax ELISA Microplate Reader. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. versamax, vmax, emax, uvmax, flexstation, softmax pro 5 gxp software, spectratest, molecular devices automation partners, gemini, lmax, assay protocols, data display, data analysis, assay. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The lysozyme concentration of a sample was determined using a standard calibration curve made of a series of known lysozyme concentrations. 2. All experiments were repeated three times. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. Finally, a 5% glucose solution was added to the suspension of CPN and FCPN as a freeze-dried protective agent. Filterless. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. After 4 h of incubation at 37 °C, culture medium was discarded and dimethyl sulfoxide (150 μL/well; Sigma) was inoculated to dissolve the precipitated formazan produced in viable cells. 1700 +1 800. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. ¨Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. 99 shipping. reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA) at 560 nm with background subtraction at 630 nm, based on which the corresponding cell viability was assessed. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Our mission: find your user guide or user's manual as quickly as possible. from Molecular Devices Corporation. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. Finally, the absorbance at 450 nm was detected by an ELISA reader, VERSAmax (Molecular Devices, San Jose, CA, USA). Cell viability was analyzed using a cell counting kit assay (Dojindo). 1. Unit price / per . For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747. reader (VersaMax, Molecular devices, San Jose, CA, USA). By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. View the supported Window operating systems for the different versions of the SoftMax Pro software. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The absorbance of each well was measured at λ = 490 nm with a VersaMax (Molecular Devices, USA) microplate reader. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Finally, the optical density of the wells was determined at an excitation wavelength of 450 nm and a reference wavelength of 540 nm on a VersaMax (Molecular Devices) microplate reader. Glossary of Terms. The SpectraTest® ABS1 Absorbance. 5 mg/mL) in the range of 5–100 μg per 0. 9 in. Select the Driver tab. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. RT-qPCR. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. 00 Add to cart Molecular Devices Microplate Reader Spectramax 384 340PC $ 6,295. And the VERSAmax reader is robotics-compatible. A solution of 10 millimolar L-DOPA (40 µL) was added to each sample and the blank. to 11. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Open the Windows Task Manager, go to the Services tab, and click Open Services. (Seongnam, Korea). The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein. A. Western Blot. After three times washing, the reaction was colored by substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (T0440, Sigma), and stopped by 2 N HCl. 9000-0756. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Glossary of Terms. ¨We would like to show you a description here but the site won’t allow us. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. +86-10-6410-8669 fax +86-10-6410-8601 Molecular Devices Shanghai Tel. 0A Sunnyvale, CA Made in USA Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The absorbance of the mixture was measured with a microplate reader (VERSAmax™, Molecular device, Sunnyvale, USA) at wavelength of 498 nm until absorbance was saturated. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). 0%, 0-2. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pm SpectraMax and VersaMax . Cell. 专为科研检测平台量身打造的灵活且强大的小型微孔板读板机（酶标仪），具备终点法和动力学检测模式，标准配置6块滤光片，覆盖蛋白定量、细胞活力、农药残留、ELISA实验等. ® The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. We would like to show you a description here but the site won’t allow us. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. A. The well plates were then washed with 1% acetic acid and dried. ChE activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction. A. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Versamax Microplate Reader dibangun di sekitar monokromator kisi. After addition of stop solution, the optical density of samples were read at wavelengths of 450 and 570 nm (Versamax, Molecular Devices Co. Lihat Video. , Sunnyvale, CA, USA). For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. 17. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. VersaMax™ Tunable Microplate Reader, Molecular Devices. 8. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Plates values were acquired using a fluorescence microplate reader (570 nm; Versamax, Molecular Devices, San Jose, CA, United States). 제조사 : Molecular Devices. All animal experiments were performed in compliance with the relevant laws and. 3. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Approximately. EUROPE. The quantity of nitrite in the samples was calculated using the concentration of sodium nitrite as a standard. With optimized reagents, option to operate the. $1,749. 747. 0, 0. A series of 44 excipients were evaluated at six different concentrations in triplicate. Ensure data accuracy of your reader. , Spectramax Gemini; Molecular Devices) Tissue culture plates (24- and 96-well) Vacuum suction pumpRelated products. Conventions Used in this Manual. Wavelength Accuracy. SpectraMax® i3 Multi-Mode Detection Platform. Spectrofluorometer (e. 9000-0756. We would like to show you a description here but the site won’t allow us. The rate of cell viability was calculated using the following formula:The conversion of NADP+ to NADPH was measured as absorbance (340 nm) on a microplate reader (VersaMax, Molecular Devices Co. +44-118-944-8000. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. P. The cells were treated with 15, 25, 30, and 45 nm-particles in order to compare the cytotoxicities of different sized nanoparticles on the treated cells. 4. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). The. End-point IgG titers were established as the mean plus three standard deviations of. The absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 450 nm. Accession codes. Supplier: Molecular Devices. for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. Nude mice were obtained from Orient Bio Inc. This robust model allows precise wavelength detection in. 1 mL. These values were transformed to logarithmic scale to create a log-log curve. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Data were analyzed with GraphPad Prism. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. ) gap between the rear of the instrument and the wall. Furnace Manuals. 4. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. 0. 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). Substrates and coenzymes provided and specified in the kit were added at 37°C after 1 min plate was read in a spectrophotometer (Versamax, Molecular Devices, San Jose, CA, USA) at 37°C every 1 min for 4 min. The TBARS content was calculated according to the extinction coefficient as 155 mM −1 cm −1. S. Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. Cet appareil ISM est confomre à la norme NMB-001 du Canada. VersaMax Microplate Reader (Molecular Devices cat. ® Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. Search for used versamax. (VersaMax Molecular Devices, Sunnyvale, CA). For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. The absorbance was measured at 450 nm using a microplate reader (VersaMax, Molecular Devices). The Indocyanine green (ICG, Sigma-Aldrich) assay measures the liver depuration capacity . Find regional support. 00 USD. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high-qualityspectrophotometer. . 8 in. Absor-bance of the solution was measured using a micro plate Reader (VersaMax, Molecular Devices) at 570 nm. The detection limit for rivaroxaban in this assay was 10 ng/mL. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. The product formed was then transferred to 96 well plate and the absorbance was measured at 540 nm using microplate reader (VersaMax™, Molecular Devices, CA, USA). Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. Monochromator, tunable in 1-nm increments. SoftMax Pro. All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. At All-usermanuals. The primers for the human genes GAPDH, SOD1, SOD2, CAT, GPX1, CCS, PRDX6, FOXO3, CDKN2A,. +81-3-5282-5261 SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. 1700 +1 800. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. 99 + $91. The reaction in the wells was stopped by the addition of 50 μl of 1 M sulfuric acid, which turned the wells yellow. For validation of the Molecular Devices VersaMax™ Absorbance Microplate Reader for use with the Pyrochrome Assay, data acquisition and analysis were performed with SoftMax ® Pro GxP Software. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). S. The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 0 out of 5 $ 2,999. Table of Contents. Patients with small intestinal neuroendocrine tumors (SI-NETs) frequently develop spread disease; however, the underlying molecular mechanisms of disease progression are not known and effective. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Results are presented as the geometric mean and are derived from at least three experiments. Molecular Devices VersaMax Absorbance (UV/Vis) Elisa Microplate Reader For Sale for Plate Reader is complete with computer, SoftMa. The cellular viability. . The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. The absorbance was measured at 570 nm using a microplate reader (VersaMax, Molecular Devices). To purchase a newer software version, request a quote. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 5 mM was used to make the. The viability of the treated group was expressed as a percentage of non-treated control group, which was assumed to be 100%. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of delivery. Our specialization servicing microplate instrumentation and our focus on a narrow range of product lines allows us to provide the highest standards of service and ensure. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. This reliable model allows for. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. 0. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. treatments were then examined atλ=570 nm using VersaMax eliSa reader (Molecular devices, llc. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. The limit of detection for each assay was 156. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. Oil red O staining. Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. Manual for Molecular Devices versamax Laboratory Equipment (47 pages) Specifications: 1379/1379020-versamax. Cell viability was represented by percentage values compared to a control and then used to calculate the number of viable cells. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. After incubation, the absorbance of the plate was measured at 450 nm using a microplate reader (VERSAmax™, Molecular Devices Corp. Regular price $ 0. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. With a wavelength range from 340 to 850 nm, it is the equivalent to having 510 free filters. Table 1. 5 million deaths worldwide have been caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (), along with high levels of unemployment and far-reaching supply chain issues in the world’s economy. 3 Methods 1. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesThe amount of extracted crystal violet was measured at 595 nm using a microplate reader (VersaMax, Molecular Devices Co. 2. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Although this manual has been prepared with every precaution to ensure accuracy, Molecular DevicesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. ChE activity was normalized by protein concentration as determined by a Coomassie. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. 0A-Sunnyvale, CA-Made in USA SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). Microplate Reader Capability > UV - Vis Absorbance. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Table 1. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). This version is compatible with the following instruments: SpectraMax® i3x Multi-Mode Detection Platform. To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. Get pricing and purchase lab equipment and. 00: Description. Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. absorbance readers. 17. The cells were seeded on the bottom of 24-well plates with sterile glass coverslips. That's why our team is at your disposal the majority of time. The index was evaluated at 48 and 72 hours normalized to that at 24 hours. MDC instruments including: SpectraMax®, VersaMax. They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 0 N sulfuric acid. StakMax Microplate Handling System. Used. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. measured over 60min using a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA) at a wavelength of 405nm. All the experiments were conducted at. Cell viability was calculated using the following formula, where OD t is the absorbance of the treated cells and OD c is the absorbance of control cells: Cell viability = O D t / O D c. The sections were stained with hematoxylin and eosin (H&E) and. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. With optimized reagents, option to operate the. Spectranet customer portalSpectraMax and VersaMax . 7. Cell survival rate of each sample was calculated as a percentage relative to cells incubated in D-MEM. SoftMax® Pro 7. Used. 8). The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochrome-based systems. (VersaMax, Molecular Devices, USA). This technical note is designed to provide information . Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到With the VersaMax microplate reader, you will never have to buy another filter. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Molecular Devices Spectramax Plate Reader. Primer design. 3, Molecular Devices). 00. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. Reboot the computer. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. After freeze-drying, the. The absorbance was detected at 517 nm wavelength using a microplate reader (VersaMax, Molecular devices, USA). Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. We would like to show you a description here but the site won’t allow us. The absorbance was measured at 540 nm using a hybrid microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). Four wash/rinse bottles are included and configurable for both 96- and 384. Color intensities were measured using microplate reader (Versamax, Molecular Devices, Inc. developing diagnostic and research tools using.